this post was submitted on 25 May 2025
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Microscopy

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Anything related to things that are too small to see them with the eye, and the tools used to observe them.

This space is quite general in scope - microscopes, microbiology, small component electronics, questions about buying optical components, etc.

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Plant stomata (mander.xyz)
submitted 1 month ago* (last edited 1 month ago) by Sal@mander.xyz to c/microscopy@mander.xyz
 

This image was taken through the 100x oil objective and a 2x camera adapter projecting the image into a Nikon D7500. The sample is a leaf from one of my plants (Dioscorea elephantipes, but I don't think this picture would look very different for other plant species)

The edges of he leaf were already yellowish brown. Here is a photo of that area with much less chlorophyll:

And here is a photo through the 40x objective using oblique illumination:

If you want to see some really fantastic photos of plant stomata I recommend having a look at Rolf Vossen's photographs here: https://microscopyofnature.com/stomata

I am looking through his documentation trying to understand how he managed to get those images. They are spectacular.

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[โ€“] Doxatek@mander.xyz 1 points 1 month ago (1 children)

Under stomata is stomatal pore into the mesophyll. Kinda weird. Here's some pics

https://propg.ifas.ufl.edu/01-biology/02-cell-types/10-celltypes-stomate.html

[โ€“] Sal@mander.xyz 2 points 1 month ago

Cool, thanks!

In this picture the cells in the epidermis layers appear to lack chloroplasts. I wonder if that's the case... That might also explain this.