This image was taken through the 100x oil objective and a 2x camera adapter projecting the image into a Nikon D7500. The sample is a leaf from one of my plants (Dioscorea elephantipes, but I don't think this picture would look very different for other plant species)
The edges of he leaf were already yellowish brown. Here is a photo of that area with much less chlorophyll:
And here is a photo through the 40x objective using oblique illumination:
If you want to see some really fantastic photos of plant stomata I recommend having a look at Rolf Vossen's photographs here: https://microscopyofnature.com/stomata
I am looking through his documentation trying to understand how he managed to get those images. They are spectacular.
If it were red it would be plant stigmata
Here ya go
Great album cover!!
Is this with a filter? A stain? Post-processing?
Thanks! Stained with saffranin
Very cool. Before staining, did you prepare it in some way?
Yeah. I was supposed to clear the leaf of cellular contents so I could see through it to the other side. Accidentally left it in acid for too long and each layer separated. This is only the epidermis one later thick
Thanks for the explanation. I will look into how to remove cellular contents and how to separate layers. I didn't know this could be achieved with acid :D
No worries. I can send you protocol
I would appreciate that!